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《医学前沿(英文)》 2015年 第9卷 第1期 页码 20-29 doi: 10.1007/s11684-014-0371-x
Ischemic stroke is a focal cerebral insult that often leads to many adverse neurological complications severely affecting the quality of life. The prevalence of stroke is increasing throughout the world, while the efficacy of current pharmacological therapies remains unclear. As a neuroregenerative therapy, the implantation of human umbilical cord mesenchymal stem cells (hUC-MSCs) has shown great possibility to restore function after stroke. This review article provides an update role of hUC-MSCs implantation in the treatment of ischemic stroke. With the unique “immunosuppressive and immunoprivilege” property, hUC-MSCs are advised to be an important candidate for allogeneic cell treatment. Nevertheless, most of the treatments are still at primary stage and not clinically feasible at the current time. Several uncertain problems, such as culture conditions, allograft rejection, and potential tumorigenicity, are the choke points in this cellular therapy. More preclinical researches and clinical studies are needed before hUC-MSCs implantation can be used as a routinely applied clinical therapy.
关键词: cellular therapy transplantation human umbilical cord mesenchymal stem cells ischemic stroke
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《医学前沿(英文)》 2013年 第7卷 第3期 页码 345-353 doi: 10.1007/s11684-013-0282-2
Human umbilical cord-derived mesenchymal stem cells (hUC-MSCs) possess various advantageous properties, including self-renewal, extended proliferation potential, multi-lineage differentiation potential and capacity for differentiating into sweat gland-like cells in certain conditions. However, little is known about the effect of clinical-grade culture conditions on these properties and on the differentiative potential of hUC-MSCs. In this study, we sought to investigate the properties of hUC-MSCs expanded with animal serum free culture media (ASFCM) in order to determine their potential for differentiation into sweat gland-like cells. We found that primary cultures of hUC-MSCs could be established with ASFCM. Moreover, cells cultured in ASFCM showed vigorous proliferation comparable to those of cells grown in classical culture conditions containing fetal bovine serum (FBS). Morphology of hUC-MSCs cultured in ASFCM was comparable to those of cells grown under classical culture conditions, and hUC-MSCs grown in both of the two culture conditions tested showed the typical antigen profile of MSCs—positive for CD29, CD44, CD90, and CD105, and negative for CD34 and CD45, as expected. Chromosomal aberration assay revealed that the cells were stable after long-term culture under both culture conditions. Like normal cultured MSCs, hUC-MSCs induced under ASFCM conditions exhibited expression of the same markers (CEA, CK14 and CK19) and developmental genes (EDA and EDAR) that are characteristic of normal sweat gland cells. Taken together, our findings indicate that the classical culture medium used to differentiate hUC-MSCs into sweat gland-like cells can be replaced safely by ASFCM for clinical purposes.
关键词: umbilical cord mesenchymal stem cells sweat gland preclinical
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《医学前沿(英文)》 2012年 第6卷 第1期 页码 41-47 doi: 10.1007/s11684-012-0175-9
Umbilical cord mesenchymal stem cells (MSCs) are a unique, accessible, and non-controversial source of early stem cells that can be readily manipulated. As the most common pluripotent cell, bone marrow-derived MSCs display limitations with the progress of stem cell therapy. By contrast, umbilical cord-derived cells, which have plentiful resources, are more accessible. However, several uncertain aspects, such as the effect of donor selection or culture conditions, long-term therapeutic effects, product consistency, and potential tumorigenicity, are the bottleneck in this clinical therapy. MSCs are predicted to undergo an unprecedented development in clinical treatment when a generally acknowledged criterion emerges. In the current paper, we highlight the application of umbilical cord-derived MSCs in skin therapies based on our previous studies, as well as the achievements of our peers in this field. This paper focuses on the strategies, challenges, and potential of this novel therapy.
关键词: umbilical cord mesenchymal stem cells cutaneous regeneration
Zheng AO, Chengfa ZHAO, Yanmin GAN, Xiao WU, Junsong SHI, Enqin ZHENG, Dewu LIU, Gengyuan CAI, Zhenfang WU, Zicong LI
《农业科学与工程前沿(英文)》 2019年 第6卷 第1期 页码 54-60 doi: 10.15302/J-FASE-2018249
Somatic cell nuclear transfer (SCNT)-derived piglets have significantly higher stillbirth rate and postnatal mortality rate than artificial insemination (AI)-generated piglets. The question whether the low survival rate of SCNT piglets was related to birth weight, umbilical cord or placenta development was investigated. In this study, stillbirth rate, neonatal death rate, birth weight, umbilical cord status, placental parameters and placental gene expression patterns were compared between SCNT and AI piglets. Results showed that mortality rates at birth and during the neonatal stage of SCNT piglets were significantly higher than those of AI piglets. The incidence of abnormal umbilical cord in SCNT and SCNT-liveborn (SCNT-LB) piglets was significantly higher than in AI and AI-liveborn (AI-LB) piglets. Birth weight, placental weight, placental surface area and placental efficiency in SCNT and SCNT-LB piglets were significantly lower than those of AI and AI-LB piglets. Placental expression profiles of imprinting, angiopoiesis and nutrient transport-related genes were defective in SCNT-LB piglets compared with those in AI-LB piglets. Thus, the low survival rate of SCNT piglets may be associated with abnormal umbilical cord and placenta development. These characteristics may have resulted from aberrant expression of angiogenesis, nutrient transport, and imprinting-related genes in the placentas.
Study on the action of resistin-induced human umbilical vein endothelial cell dysfunction
LI Zhizhen, LI Fangping, YAN Li, LI Feng, LI Yan, CHENG Hua, FU Zuzhi
《医学前沿(英文)》 2007年 第1卷 第2期 页码 196-199 doi: 10.1007/s11684-007-0037-z
关键词: endothelial resistin stimulation Incubation pathogenesis dysfunction
Chengwei JING, Qin FU, Xiaojun XU
《医学前沿(英文)》 2009年 第3卷 第1期 页码 45-48 doi: 10.1007/s11684-009-0019-4
关键词: central cord syndrome decompression surgical titanium mesh
Bin Yu, Xiaosong Gu
《医学前沿(英文)》 2019年 第13卷 第2期 页码 131-137 doi: 10.1007/s11684-018-0642-z
关键词: spinal cord injury biomaterial extrinsic barrier intrinsic regeneration capacity
Primary spinal epidural non-Hodgkin’s lymphoma presented with spinal cord compression syndrome
Chunquan CAI MD, PhD, Qingjiang ZHANG BM, Changhong SHEN MD, PhD,
《医学前沿(英文)》 2009年 第3卷 第4期 页码 499-502 doi: 10.1007/s11684-009-0075-9
关键词: non-Hodgkin’ s lymphoma primary spinal cord compression syndrome epidural space
Developmental expression of CAPON and Dexras1 in spinal cord of rats
LI Xin, CHENG Chun, CHEN Mengling, GAO Shangfeng, ZHAO Jian, NIU Shuqiong, SHEN Aiguo
《医学前沿(英文)》 2008年 第2卷 第1期 页码 75-81 doi: 10.1007/s11684-008-0014-1
关键词: CAPON ISH differentiation postnatal different subcellular
Prevention of laryngeal webs through endoscopic keel placement for bilateral vocal cord lesions
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《医学前沿(英文)》 2018年 第12卷 第3期 页码 301-306 doi: 10.1007/s11684-017-0549-0
Transoral microresection for treatment of vocal cord lesions involving the anterior commissure may result in anterior glottic webs. In this study, we retrospectively reviewed 54 patients who underwent microsurgery for bilateral lesions involving the anterior commissure and categorized them into two groups. The keel placement and control groups received endoscopic keel placement and mitomycin C, respectively. During the follow-up of at least 1 year, the laryngeal web formation rate significantly decreased in the keel placement group compared with that in the control group (18.6% versus 54.5%, <0.05). Furthermore, the voice handicap index-10 scores for patients without web formation decreased in both the keel placement and control groups ( <0.0001 and <0.001, respectively). A pseudomembrane covering the vocal cords was detected in 16.3% (7 of 43) cases after keel removal. A total of 100% (7 of 7) of these cases and 2.8% (1 of 36) of the other cases formed laryngeal webs ( <0.0001). Endoscopic keel placement could be an effective method for preventing anterior glottic webs after surgery for bilateral vocal cord diseases involving the anterior commissure. The pseudomembrane observed at the time of keel removal may imply a high risk of web formation.
关键词: glottic stenosis glottic web laryngeal keel silastic sheets anterior commissure
梅群,沈兆武,周听清
《中国工程科学》 2005年 第7卷 第6期 页码 69-72
系统地研究了超低能导爆索的传爆原理,结合工程实际需要,拓宽应用范围的研究,为深孔土岩爆破的反向起爆提出一种新型的,安全、可靠、高效的起爆网络。
Challenges of human–machine collaboration in risky decision-making
《工程管理前沿(英文)》 2022年 第9卷 第1期 页码 89-103 doi: 10.1007/s42524-021-0182-0
关键词: human–machine collaboration risky decision-making human–machine team and interaction task allocation human–machine relationship
Zooming in and out of ferroptosis in human disease
《医学前沿(英文)》 2023年 第17卷 第2期 页码 173-206 doi: 10.1007/s11684-023-0992-z
关键词: ferroptosis human disease iron metabolism lipid peroxidation antioxidation
Dan DAI, Jing LIU
《能源前沿(英文)》 2012年 第6卷 第3期 页码 210-226 doi: 10.1007/s11708-012-0200-3
关键词: human energy harvesting human-powered transportation human-powered home appliances human-powered grid (HPG) human-powered charger (HPC) human-powered storage (HPS) biofuel
标题 作者 时间 类型 操作
Implantation of human umbilical cord mesenchymal stem cells for ischemic stroke: perspectives and challenges
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期刊论文
Capacity of human umbilical cord-derived mesenchymal stem cells to differentiate into sweat gland-like
null
期刊论文
Umbilical cord-derived mesenchymal stem cells: strategies, challenges, and potential for cutaneous regeneration
null
期刊论文
Comparison of birth weight and umbilical and placental characteristics of cloned and artificial insemination-derived
Zheng AO, Chengfa ZHAO, Yanmin GAN, Xiao WU, Junsong SHI, Enqin ZHENG, Dewu LIU, Gengyuan CAI, Zhenfang WU, Zicong LI
期刊论文
Study on the action of resistin-induced human umbilical vein endothelial cell dysfunction
LI Zhizhen, LI Fangping, YAN Li, LI Feng, LI Yan, CHENG Hua, FU Zuzhi
期刊论文
Anterior cervical surgery methods for central cord syndrome without radiographic spinal fracture-dislocation
Chengwei JING, Qin FU, Xiaojun XU
期刊论文
and genetic enhancement of intrinsic growth capacities to promote CNS axon regeneration after spinal cord
Bin Yu, Xiaosong Gu
期刊论文
Primary spinal epidural non-Hodgkin’s lymphoma presented with spinal cord compression syndrome
Chunquan CAI MD, PhD, Qingjiang ZHANG BM, Changhong SHEN MD, PhD,
期刊论文
Developmental expression of CAPON and Dexras1 in spinal cord of rats
LI Xin, CHENG Chun, CHEN Mengling, GAO Shangfeng, ZHAO Jian, NIU Shuqiong, SHEN Aiguo
期刊论文
Prevention of laryngeal webs through endoscopic keel placement for bilateral vocal cord lesions
null
期刊论文